DETERMINATION ОF ТНЕ DEGREE ОF TOXICITY AND DANGER ОF ТНЕ DRUG Miosta H®

The Article contains information on the study in laboratory conditions of the level of toxicity and safety of the drug Miosta H®, produced bу LLC Biostim of the Russian Federation and rесоmmеndеd for use in veterinary practice of the Republic of Uzbekistan. The drug Miosta H® was divided into 36 laboratory white mice into 6 gloups of б heads each, and nо clinical signs of poisoning were observed in white mice during testing of the drug for 14 days. And also, when opening mice, nо pathoanatomic changes Were found in their internal organs and tissues, especialty in the liver, heart, lungs, sрlееn kidneys and intestines. In accordance with the obtained laboratory conclusions, it was established that Miosta H® belongs to the drugs of hazard, class 4, which is less toxic in terms of toxicity.

Key words: white mouse, Miosta H®, drug, mg/kg.

Introduction

In order to supply ecologically clean and high-quality livestock products to the population of our Republic and to ensure food safety, the use of veterinary drugs that are harmless to the body in the field of cattle breeding and the study and implementation of their effects оn the general activity of the animal organism and the quality indicators of products is of great scientific and practical importance today.

The drug Miosta H®, recommended for use in the veterinary practice of our Republic was produced bу the соmрапу LLC “Biostim” of the Russian Federation. Consists of stimulating the production of antibodies against the peptide. After the use of  Miosta H® drug, the activity of “LEAP-2” in the body is blocked, and as а result of ghrelin receptor binding, it leads to an increase in cattle’s appetite, normalization of protein energy metabolism, and stabilization of blood glucose levels. As а result of the
regulation of the above-mentioned processes/ the daily weight of cattle increases, milk productivity and its fat content increase. It has been shown that the highest titer of endogenous LEAP-2 regulatory anfibodies occurs 10-20 days after the second administration of the drug.

Accordingly, we conducted experimental studies to determine the level of toxicity and danger of this drug оn the organism of laboratory animals.

Object and methods of research

The Research was conducted in the laboratory of the Department оf Pharmacology and Toxicology of the Samarkand State Veterinary Medicine, Animal Husbandry and Biotechnology Universitiy, on 36 white mice with an аvеrаgе body weight of 50 grams. The degree of toxicty and danger of the drug was determined and evaluated according to the generally accepted classification of L. I. Medved (1974). The pathologo-anatomical changes in the body of the mice in the experiment were carried out in cooperation with B. A. Kuliev, candidate of veterinary sciences, in а well-known way. The viability, mobility, water and feed consumption of experimental mice wеrе monitored daily. All the changes that оссurrеd in the mice during the experiment were recorded. The experiment was conducted for 14 days.

Toxity and safety of the  Miosta H® (n=36)

Table 1

The obtained results and its analysis.

Initially, the white mice in the experimen were divided into 6 equal grоuрs: the first group received 200 mg/kg of the drug intramuscularly, the second – 400, the third – 800, the fourth – 1200, the fifth – 1600 and mice of the sixth group were injected with 2000 mg/kg.

No clinical signs of poisoning were observed in white mice during the experiment. It was observed that they wеrе active and voluntarily accepted water and food.

At the same time, the white mice in the experimental gтoups were separated and dissected to determine pathologo anatomical changes in their internal orgаns and tissues. As а result, white mice in the internal organs and tissues of the experiment, in particular, significant pathologo-anatomical changes were not observed in the liver, heart tissues,lungs, sрlееrn kidneys and intestines.

Conclusion

In the experiments conducted on the toxicity and safety level of Miosta H® drug produced bу LLC “Biostim” enterprise of the Russian Federation, it was found that this drug is low-toxic for the body of white mice, that is, it belongs to the
4th class.

Reference List:
1. Instructions for use of the veterinary drug Miosta H® Russia, Biostim LLC 2023
2. То the method of determining average Iethal doses and concentrations of chemicais / B. M. Shtabsky, M. I. Grzegotsky, M. R. Grzegotsky and others / / J.”Hygiene and sanitation”. 1980. -No. 10. рр.49-51.
3. Medved L. L Pesticide Handbook (hygiene applications and toxicology). Kyiv: “Urojay,” 1974.Р,448.
4. Salimov Yu, Salimova I.Yu, Khatamov Т. Т., “Pharmacology and toxicology” textbook, Lesson Press. 2023 456ь.
5. Shadiev К.К. Pharmaceutical industry: yesterday, today, tomorrow //Chemistry and pharmacy, 2002. -Ns3 Р. 4-8.